Straightforward and Accurate Genotyping-by-Sequencing

powered by Data2Bio's tGBS® genotyping-by-sequencing (GBS) technology

Ott A, S Liu, JC Schnable, CT Yeh, KS Wang, PS Schnable (2017) tGBS® genotyping-by-sequencing enables reliable genotyping of heterozygous loci. Nucleic Acids Res, 45(21): e178

View Article

Genotyping Pricing for Plant Species

Basic

$30 / sample1,4
Up to 2,000 segregating SNPs2
Requires existing reference genome

Advanced

$35 / sample1,4
2,000-10,000 segregating SNPs2
Requires existing reference genome
Perfect for mapping QTLs or single segregating genes in biparental, NAM, or MAGIC populations

Comprehensive

$50 / sample1,3,4
10,000-100,000+ segregating SNPs2
Does not require existing reference genome
Suitable for genotyping association panels for subsequent GWAS or designing genotyping arrays

Features

Basic Genotyping Features

  • Genotyping-by-sequencing for plant species
  • Requires existing reference genome
  • Sample QC
  • Library preparation and sequencing
  • SNP variants (VCF) and allele counts (Up to 2,000 segregating SNPs2)
  • Automated customer service
  • Standard turn around time of 20 weeks5
  • Data retention/backup of 2 weeks
  • Online download of results
  • Minimum of 192 samples per order

Advanced Genotyping Features

  • Genotyping-by-sequencing for plant species
  • Requires existing reference genome
  • Sample QC
  • Library preparation and ssequencing
  • SNP variants (VCF) and allele counts (2,000-10,000 segregating SNPs2)
  • Individual FASTQ files of each sample
  • BAM (alignment) files of each sample
  • Automated customer service
  • Standard turn around time of 10-14 weeks5
  • Rush processing option available (turn around time of 8 weeks6)
  • Data retention/backup of 1 month
  • Online download and/or mailed hard drive7 of results
  • Minimum of 96 samples per order

Comprehensive Genotyping Features

  • Genotyping-by-sequencing for plant species
  • Does NOT require existing reference genome
  • Sample QC
  • Library preparation and sequencing
  • Reference-free genome generation when no reference genome is available
  • SNP variants (VCF) and allele counts (10,000-100,000+ segregating SNPs2)
  • Individual FASTQ files of each sample
  • BAM (alignment) files of each sample
  • Project manager customer service
  • Standard turn around time of 10-14 weeks5
  • Rush processing option available (turn around time of 8 weeks6)
  • Data retention/backup of 2 months
  • Online download and/or mailed hard drive7 of results
  • Minimum of 96 samples per order
  Basic Advanced Comprehensive
Supported Species Plants Plants Plants
Requires Existing Reference Genome Yes Yes Optional
Sample QC ✓ ✓ ✓
Library Preparation ✓ ✓ ✓
Sequencing ✓ ✓ ✓
SNP Variants (VCF) and Allele Counts ✓ ✓ ✓
Individual FASTQ Files Provided   ✓ ✓
BAM (Alignment) Files Provided   ✓ ✓
Number of Segregating SNPs2 Up to 2,000 2,000 ~ 10,000 10,000 ~ 100,000+
Customer Service Automated Automated Project Manager
Standard Turn Around Time5 20 Weeks 10-14 Weeks 10-14 Weeks
Rush Processing Available6   8 Weeks 8 Weeks
Data Retention/Backup 2 Weeks 1 Month 2 Months
Results Delivery Online Download Only Online Download
and/or Mailed Hard Drive7		 Online Download
and/or Mailed Hard Drive7
Minimum Number of Samples 192 96 96

1 Species with genomes >5 GB may incur additional costs

2 Exact number of segregating SNPs contingent on sequence diversity among genotyped samples and quality of reference genome

3 Additional cost of $20/sample for species without a reference

4 Researchers based in any of these countries are eligible for discounted pricing in the form of credit towards future projects

5 Standard turn-around time is estimated after samples pass QC

6 Additional cost of $10/sample for rush processing

7 A flat fee of $150 for delivery of final results on a mailed hard drive. The delivered hard drive is yours to keep

Download Sample Project Report

Interested?

If you are ready to start a project, please fill out the request a quote form and you will receive instructions on how to submit your samples.

Not quite ready yet? Fill out the form below to receive updates and special offers from Freedom Markers in the future.