:: Freedom Markers - How it Works ::

Featured tGBS^® Papers

ALLMAPS: robust scaffold ordering based on multiple maps

Genetic characterization of maximilian sunflower for the development of a locally adapted perennial grain oilseed

Phenotypic data from inbred parents can improve genomic prediction in pearl millet hybrids

Genomic analysis reveals multiple mismatches between biological and management units in yellowfin tuna (Thunnus albacares)

Detection of genomic loci associated with chromosomal recombination using high-density linkage mapping in Setaria

The conservation genomics of the endangered distylous gypsophile Oreocarya crassipes (Boraginaceae)

Conservation genetics of Phlox hirsuta, a serpentine endemic

Integration of Genetic and Cytogenetic Maps and Identification of Sex Chromosome in Garden Asparagus (Asparagus officinalis L.)

Genome-wide analyses reveal footprints of divergent selection and popping-related traits in CIMMYT's maize inbred lines

Genome-wide association mapping reveals loci for shelf life and developmental rate of lettuce

Molecular markers reliably predict postharvest deterioration of fresh-cut lettuce in modified atmosphere packaging

Construction of a dense genetic map of the Malus fusca fire blight resistant accession MAL0045 using tunable genotyping-by-sequencing SNPs and microsatellites

Simultaneous Improvement and Genetic Dissection of Salt Tolerance of Rice (Oryza sativa L.) by Designed QTL Pyramiding

tGBS^® genotyping-by-sequencing enables reliable genotyping of heterozygous loci

Identifying loci with breeding potential across temperate and tropical adaptation via EigenGWAS and EnvGWAS

Genetic Determinants of Crop Timing and Quality Traits in Two Interspecific Petunia Recombinant Inbred Line Populations

Detection, Validation, and Application of Genotyping-by-Sequencing Based Single Nucleotide Polymorphisms in Upland Cotton

Genetic characterization of inbred lines from Shaan A and B groups for identifying loci associated with maize grain yield

Exploiting the genomic diversity of rice (Oryza sativa L.): SNP-typing in 11 early-backcross introgression-breeding populations

Genetic diversity, population structure, and botanical variety of 320 global peanut accessions revealed through tunable genotyping-by-sequencing

Genotyping-by-sequencing reveals three QTL for clubroot resistance to six pathotypes of Plasmodiophora brassicae in Brassica rapa

Genetic diversity assessment of Georgia peanut cultivars developed during ninety years of breeding

Development of reduced gluten wheat enabled by determination of the genetic basis of the lys3a low hordein barley mutant

Identification of loci controlling adaptation in chinese soybean landraces via a combination of conventional and bioclimatic GWAS

Development of decreased-gluten wheat enabled by determination of the genetic basis of lys3a barley

How it Works

Freedom Markers specializes in tGBS^® genotyping-by-sequencing (GBS) for plant species. With over thirty years combined experience, we have found ways to optimize the process to increase speed, reduce cost, and improve the quality of the final results.

The first step to producing the highest quality genotyping results is to start with DNA of extremely consistent and high quality across samples. For this reason, Freedom Markers offers in-house DNA extractions to all customers at no additional charge. If you select this option, tissue collection instructions and supplies will be shipped to you after the quote has been accepted and a purchase order (PO) is on file. It is recommended that samples be lyophilized (freeze dried) to allow shipment at room temperature. However, if a lyophilizer is not available, the alternative method is to ship fresh samples overnight, packed with cooling packs from a refrigerator. Please do NOT send samples on ice, dry ice, or cooling packs from a freezer, as these may freeze the tissue samples. The Freedom Markers facility is unable to process frozen tissue samples, and any plates received with frozen samples will have to be discarded. When samples are received by Freedom Markers, DNA isolation will be performed using optimized, high-throughput protocols at the facility.

In order to test and optimize DNA extraction before working with your irreplaceable experimental samples, please include a ziplock bag with additional tissue collected from the species at the same stage of growth. Whenever possible, it is recommend that customers submit two replicates of tissue for DNA extraction. This allows genotyping to continue with minimum hassle or delay if unforeseen difficulties arise during DNA isolation. However, we understand that it is sometimes not possible to collect duplicate samples.

In cases where new tissue cannot be collected, Freedom Markers will accept previously extracted DNA. However, customers should be aware that the quality of the resulting genotype calls may be lower, and the lab reserves the right to cancel any genotyping project -- at no charge to the customer -- in cases where the QC of DNA samples indicates Freedom Markers is unlikely to be unable to deliver quality final results.

After DNA is extracted and QC'd, library construction and sequencing are performed. Next alignments and SNP calls are generated. Finally, clients will receive an e-mail, containing the final project report and either download instructions for all supporting data and SNP calls or a tracking number for a hard drive containing these data.

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Freedom Markers is a provider of genotyping services powered by Data2Bio technology. This page is intended to serve as notice under 35 U.S.C. § 287(a).

The following U.S. patents apply to Data2Bio technologies, including but not limited to technologies related to tGBS^® genotyping-by-sequencing (GBS) services:

Patent Number(s): US 9,951,384

Other US and international patents are pending.